NASC

N150332

Name: AT_8088

Price: £11.00

Donor John Innes Centre Caroline Dean

Stock type: individual line

Material type: seed

Description Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.

Phenotype Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.

N150333

Name: AT_5048

Price: £11.00

Donor John Innes Centre Caroline Dean

Stock type: individual line

Material type: seed

Description Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.

Phenotype Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.

N150334

Name: AT_8085

Price: £11.00

Donor John Innes Centre Caroline Dean

Stock type: individual line

Material type: seed

Description Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.

Phenotype Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.

N150335

Name: AT_10035

Price: £11.00

Donor John Innes Centre Caroline Dean

Stock type: individual line

Material type: seed

Description Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.

Phenotype Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.

N150336

Name: AT_13424

Price: £11.00

Donor John Innes Centre Caroline Dean

Stock type: individual line

Material type: seed

Description Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.

Phenotype Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.

N150337

Name: AT_8032

Price: £11.00

Donor John Innes Centre Caroline Dean

Stock type: individual line

Material type: seed

Description Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.

Phenotype Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.

N150338

Name: AT_8050

Price: £11.00

Donor John Innes Centre Caroline Dean

Stock type: individual line

Material type: seed

Description Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.

Phenotype Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.

N150339

Name: AT_8054

Price: £11.00

Donor John Innes Centre Caroline Dean

Stock type: individual line

Material type: seed

Description Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.

Phenotype Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.

N150340

Name: AT_8015

Price: £11.00

Donor John Innes Centre Caroline Dean

Stock type: individual line

Material type: seed

Description Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.

Phenotype Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.

N150341

Name: AT_8030

Price: £11.00

Donor John Innes Centre Caroline Dean

Stock type: individual line

Material type: seed

Description Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.

Phenotype Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.

N150342

Name: AT_8023

Price: £11.00

Donor John Innes Centre Caroline Dean

Stock type: individual line

Material type: seed

Description Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.

Phenotype Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.

N150343

Name: AT_10036

Price: £11.00

Donor John Innes Centre Caroline Dean

Stock type: individual line

Material type: seed

Description Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.

Phenotype Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.

N150344

Name: AT_8051

Price: £11.00

Donor John Innes Centre Caroline Dean

Stock type: individual line

Material type: seed

Description Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.

Phenotype Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.

N150345

Name: AT_8072

Price: £11.00

Donor John Innes Centre Caroline Dean

Stock type: individual line

Material type: seed

Description Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.

Phenotype Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.

N150346

Name: AT_4039

Price: £11.00

Donor John Innes Centre Caroline Dean

Stock type: individual line

Material type: seed

Description Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.

Phenotype Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.

N150347

Name: AT_4133

Price: £11.00

Donor John Innes Centre Caroline Dean

Stock type: individual line

Material type: seed

Description Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.

Phenotype Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.

N150348

Name: AT_4117

Price: £11.00

Donor John Innes Centre Caroline Dean

Stock type: individual line

Material type: seed

Description Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.

Phenotype Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.

N150349

Name: AT_4095

Price: £11.00

Donor John Innes Centre Caroline Dean

Stock type: individual line

Material type: seed

Description Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.

Phenotype Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.

N150350

Name: AT_5066

Price: £11.00

Donor John Innes Centre Caroline Dean

Stock type: individual line

Material type: seed

Description Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.

Phenotype Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.

N150351

Name: AT_5056

Price: £11.00

Donor John Innes Centre Caroline Dean

Stock type: individual line

Material type: seed

Description Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.

Phenotype Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.

N150352

Name: AT_10189

Price: £11.00

Donor John Innes Centre Caroline Dean

Stock type: individual line

Material type: seed

Description Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.

Phenotype Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.

N150353

Name: AT_10027

Price: £11.00

Donor John Innes Centre Caroline Dean

Stock type: individual line

Material type: seed

Description Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR as described on the protocol page (See JIC Activate collection page). This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.

Phenotype Ds activation tagged lines. Flanking sequences were obtained from transposon lines by IPCR or TAIL PCR from the 5' and 3' end of the AT-Ds (in many cases flanking sequence will only have been obtained from one end due to fragment length). All lines supplied should be carefully checked by PCR. This will confirm that the insertion is carried within the line supplied, confirm if the line is homozygous or heterozygous for the insertion, and provide DNA templates for sequencing the ends of the transposon (confirm that the 200bp inverted repeats at the end of Ds are intact). Most importantly this will ensure that you do not waste time in the event that the insertion is not maintained.